Title page for etd-0631114-104856


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URN etd-0631114-104856
Author Hsin-Pai Lee
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2013
Semester 2
Degree Ph.D.
Type of Document
Language English
Title The anti-inflammatory effects of lemnalol on
monosodium urate crystal-induced arthritis in rats
Date of Defense 2014-07-03
Page Count 133
Keyword
  • osteoclast
  • COX-2
  • iNOS
  • inflammation
  • lemnalol
  • gout
  • Abstract An acute gout attack manifests in the joint as dramatic inflammation. To date, the clinical use of medicinal agents has typically led to undesirable side effects. Numerous efforts have failed to create an effective and safe agent for the treatment of gout. Lemnalol—an extract from Formosan soft coral—has documented anti-inflammatory and anti-nociceptive properties. We attempt to examine the therapeutic effects of lemnalol on intra-articular monosodium urate (MSU)-induced gouty arthritis in rats and found that treatment with lemnalol (intramuscular [im]) significantly attenuated MUS-induced mechanical allodynia, paw edema, and knee swelling. Histomorphometric and immunohistochemistry analysis revealed that MSU-induced inflammatory cell infiltration as well as the elevated expression of c-Fos and pro-inflammatory proteins (inducible nitric oxide synthase and cyclooxygenase-2) observed in synovial tissue were significantly inhibited by treatment with lemnalol. Moreover, we also investigated the effects of lemnalol, on mast cells function and osteoclast activities in gout arthritis. We found that both infiltration and degranulation of mast cells increased at 24 h after MSU injection in the ankle joint. Immunohistochemical analysis showed that MSU induced upregulation of TGF-β1 and MMP-9 and the osteoclast markers cathepsin K and TRAP in ankle tissues. We conclude that lemnalol treatment may be beneficial for improving gouty arthritis symptoms and signs, and attenuation the inflammatory reaction including neutrophils, mast cells infiltration and mast cell degranulation and for suppressing osteoclast activation in gouty arthritis. Lemnalol may be a promising candidate for the development of a new treatment for gout and other acute inflammatory arthritis diseases.
    Advisory Committee
  • Ming-Hong Tai - chair
  • Chang-Yi, Wu - co-chair
  • Hui-Min Wang - co-chair
  • Yen-Hsuan Jean - co-chair
  • Zhi-hong, Wen - advisor
  • Files
  • etd-0631114-104856.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2014-07-31

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