Title page for etd-0630113-014657


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URN etd-0630113-014657
Author Cheng-yang Kang
Author's Email Address No Public.
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Department Marine Biology
Year 2012
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Studies on the Enhancement of Chilling Tolerance of Rice
(Oryza sativa L.) Seedlings by Overexpression of Glutathione
Reductase from Marine Macroalga Ulva fasciata Delile
Date of Defense 2013-05-22
Page Count 54
Keyword
  • Oryza sativa
  • GSH (reduced glutathione)
  • GR (Glutathione reductase)
  • transformation
  • oxidative stress
  • salt stress
  • low temperature stress
  • Abstract The purpose of this study is to construct the gene of glutathione reductase (UfGr) of marine macroalga Ulva fascitat Delile following with GFP gene in Agrobacterium Ti vector and transformed to Oryza sativa L. cv. Tainung 67 (TNG67) to test whether overexpression of UfGr could enhance stress tolerance (low temperature and salt stress) in rice seedlings. The GFP green fluorescence and GR activity showed there are 3 transgenic lines, OE-UfGR-1, OE-UfGR-3, and OE-UfGR-16. Using 4-leaf age seedlings, OE-UfGR-1 is tolerant to low temperature (4oC) and salt stress (200 mM NaCl), OE-UfGR-3 is tolerant to salt stress, but OE-UfGR-16 did not tolerate low temperature and NaCl stress. The physiological analysis of OE-UfGR-1 to low temperature stress showed that OE-UfGR-1 seedlings maintained higher GR activity and glutathione (GSH)/oxidized glutathione (GSSG) ratio while malondialdehyde (MDA as lipid peroxidation marker) contents are low as compared to wild type. The activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) of OE-UfGR-1 seedlings was not affected after low temperature stress.It is expected that OE-UfGR-1 seedlings effectively reduce GSSG to GSH and in turn, results in a higher reducing potential for the enhancement of low temperature tolerance.It is needed to be proven in the future. In conclusion, this study shows that overexpression of UfGr could enhance the tolerance of rice seedlings to low temperature stress.
    Advisory Committee
  • Chwan-Yang Hong - chair
  • Yi-Ting Hsu - co-chair
  • Tse-Min Lee - advisor
  • Files
  • etd-0630113-014657.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2013-07-30

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