Title page for etd-0630112-151110


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URN etd-0630112-151110
Author Jing-lian Wu
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2011
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Diversity of Endosymbiotic Bacteria of the Sponge, Cinachyrella australiensis
Date of Defense 2012-06-15
Page Count 54
Keyword
  • cryo-section
  • Fluorescent in situ hybridization(FISH)
  • Cinachyrella australinesis
  • bacteria symbiont
  • Abstract Sponge are primitive multi-cellular organisms. They are important sources of secondary metabolites. In the previous studies indicated that the sponges harbor stable symbiotic microbial consortia. The mechanisms for maintenance and transmission of microbial consortia to the next generations are still not fully understood. The sponge, Cinachyrella australinesis, was chosen to further investigate relationship of the symbiotic bacteria within to the host. Fluorescent in situ hybridization (FISH)was employed with non-specific (EUB338)and specific oligonucleotide probes for bacteria. The sponge was cryo-sectioned(1μm)and hybridized with fluorescent probes. The distribution and ratios of the bacteria in the sponge agreed with those of previous studies indicating that the symbiotic bacteria of C. australiensis are stable and endosymbiotic in nature.
    Advisory Committee
  • Chan-Shing Lin - chair
  • Yang-Yih Chen - co-chair
  • Chi-Hsin Hsu - advisor
  • Files
  • etd-0630112-151110.pdf
  • Indicate in-campus at 1 year and off-campus access at 1 year.
    Date of Submission 2012-06-30

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