Title page for etd-0628116-114955


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URN etd-0628116-114955
Author Yu-wei Li
Author's Email Address M032050007@student.nsysu.edu.tw
Statistics This thesis had been viewed 5570 times. Download 56 times.
Department Institute of Biomedical Sciences
Year 2015
Semester 2
Degree Master
Type of Document
Language English
Title The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice
Date of Defense 2016-07-27
Page Count 83
Keyword
  • Mouse model
  • Glioma
  • YAP1
  • MST1/2
  • Hippo pathway
  • Glioblastoma
  • Apoptosis
  • Verteporfin
  • Abstract Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor. Primary glioblastomas have a worse prognosis with a median survival of less than 2 years after diagnosis. The Hippo signaling transduction pathway plays multiple functions during organ development, adult tissues homeostasis and tumorigenesis, and its downstream mediator yes-associated protein 1 (YAP1) is known to function as a potent oncogene, which is amplified and overexpressed in various human cancers. The goal of this study is to study the role of Hippo pathway in GBM carcinogenesis. First, we selected primary murine glioblastoma cancer cell lines as an in vitro model system to study the functional roles of Hippo pathway in regulating the proliferation, adhesion and migration of GBM cells in vitro. Second, to identify the role of Hippo pathway in brain development, we selected GFAP drive Cre transgenic system to cross with conditional Mst1/Mst2 loxp mice to specific knockout Mst1/Mst2 (upstream kinases of Hippo pathway) expression in the glial cells during brain development. Moreover, to discovery the important function of the Hippo signaling involved in the development of GBM, we have established mouse models of GBM (activation of KrasG12D combined with p53 loss) for studying the critical roles of Hippo pathway in GBM progression. An understanding of the temporal regulation of Hippo during GBM progression and the identification of activation Hippo signaling combined p53 induced a transcriptional profiles— with respect to upstream activators and downstream networks, which may play an important node in the design of clinical approaches targeting Hippo signaling for the treatment of GBM.
    Advisory Committee
  • Hung-Wen Huamg - chair
  • Chen, Chun-Lin - co-chair
  • Kuang-hung Cheng - advisor
  • Files
  • etd-0628116-114955.pdf
  • Indicate in-campus at 2 year and off-campus access at 2 year.
    Date of Submission 2016-07-28

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