Title page for etd-0627116-141935


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URN etd-0627116-141935
Author Ke Hsuan Yen
Author's Email Address No Public.
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Department Chemistry
Year 2015
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Determination of Inorganic arsenic compound in rice and seaweed by HPLC-DRC-CVG-ICP-MS
Date of Defense 2016-07-21
Page Count 109
Keyword
  • HPLC
  • ICP-MS
  • CVG
  • DRC
  • Abstract In the first study, we assess the inorganic arsenic species content in seaweed and rice. The determination of arsenic species have bee used ion exchange chromatography on an anion exchange column with inductively coupled plasma mass spectrometry detection after microwave assisted extraction. The arsenic species studied were arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsonic acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC). Chromatographic separation of all the species was achieved 6 min in gradient elution mode using (NH4)2CO3 and methanol at pH 8.5. The recovery of inorganic arsenic species with using ultra-pure water in seaweeds were up to 100%. The limits of detection were in the range of 0.005-0.008 ng mL-1 for various arsenic species based on peak area.
     In the Second study is a method employing a chemical vapor generation system and LC combines with inductively coupled plasma mass spectrometry (CVG-LC-ICP-MS) is presented for the determination of arsenic species in rice sample. To get best signal, we have tested concentration of L-cysteine, HCl and NaBH4. Because we have used HCl as acidifying agent. To avoid 40Ar35Cl interferes with 75As, we choose O2 as collision gas in DRC system to aviod polyatomic ions 40Ar35Cl + and get the lower detection limit.The accuracy of the method is evaluated by analyzing by analyzing rice flour certified reference material (SRM 1568a).The method has been applied on rice sample.
    Advisory Committee
  • Yeou-Lih Huang - chair
  • Yen-Ling Chen - co-chair
  • S.-J. Jiang - advisor
  • Files
  • etd-0627116-141935.pdf
  • Indicate in-campus at 3 year and off-campus access at 3 year.
    Date of Submission 2016-07-27

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