Title page for etd-0626107-112159


[Back to Results | New Search]

URN etd-0626107-112159
Author Wen-hung Huang
Author's Email Address am22587@yahoo.com
Statistics This thesis had been viewed 5585 times. Download 4 times.
Department Biological Sciences
Year 2006
Semester 2
Degree Master
Type of Document
Language English
Title Effect and mechanism of 6-OHDA induced inflammation in rat urinary bladder and prostate
Date of Defense 2007-06-05
Page Count 51
Keyword
  • plasma leakage
  • 6-OHDA
  • inflammatory response
  • prostate
  • urinary bladder
  • Abstract The mechanisms underlying the 6-hydroxydopamine (6-OHDA)-induced inflammatory response in the urinary bladder and prostate in anaesthetized male rats of Long- Evan strain were investigated. The magnitude of inflammatory responses were evaluated by morphometric analysis of the area density of India ink-labeled blood vessels in urinary bladder whole mounts and spectrophotometric analysis of Evans blue dye contents in urinary bladder and prostate. Moreover, scanning electron microscopy was employed to observe the venular endothelium in the urinary bladder wall and glandular epithelium in the prostate gland. Fifteen minutes after local application of 6-OHDA to the urinary bladder, 6-OHDA induced an increase of plasma leakage in a dose-dependent manner. It was revealed that area densities of India ink-labeled blood vessels in the rat urinary bladder whole mount were 5.65±1.72 % (N=6), 22.63±3.12 % (N=6), and 35.02±2.25 % (N=6) respectively, following a local injection of vehicle, 5 mg/kg 6-OHDA, and 10 mg/kg 6-OHDA. Using Evans blue dye as a tracer for spectrophotometric analysis, the results were similar. The Evans blue dye content was 80.53±60.74 ng/mg in the urinary bladder and 48.81±2.83 ng/mg in the prostate following injection of 5 mg/kg 6-OHDA (N=6). The Evans blue dye content was 157.73±4.45 ng/mg in the bladder and 65.52±4.25 ng/mg in the prostate following injection of 10 mg/kg 6-OHDA (N=6). Evans blue dye contents in the vehicle group (N=6) were much lower, 18.82±3.74 ng/mg in the urinary bladder and 18.50±2.47 ng/mg in the prostate, which were significantly smaller than the 6-OHDA treated group. Interestingly, the inflammatory responses were completely abolished by pretreating alone with dimethylthiourea (DMTU), a hydroxyl radical scavenger, and were moderately attenuated by pretreatment with L-732,138, a NK1 receptor antagonist. Under scanning electron microscope observation, 6-OHDA caused endothelial gaps formation in the venules of urinary bladder wall and triggered the release of secretory granules in the prostate gland cells. We concluded that 6-OHDA could induce inflammation in the urinary bladder and prostate gland involving free radical and tachykinin mechanisms.
    Advisory Committee
  • Shiping He - chair
  • Tai,Ming-Hong - co-chair
  • Huang, Hung-Tu - advisor
  • Files
  • etd-0626107-112159.pdf
  • indicate in-campus access in a year and off_campus not accessible
    Date of Submission 2007-06-26

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys