Title page for etd-0623118-161304


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URN etd-0623118-161304
Author Wei-Han Hung
Author's Email Address No Public.
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Department Institute of Medical Science and Technology
Year 2017
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Differentiation of esophageal cancer tissues by polarization-resolved two-photon microscopy
Date of Defense 2018-07-23
Page Count 72
Keyword
  • esophageal cancer
  • two-photon excited florescence
  • two-photon microscopy
  • second harmonic generation
  • polarization
  • Abstract Esophageal cancer has fatally attacked human health over the past few decades. It is often diagnosed at a late stage due to how difficult detecting early cancerous changes when using conventional endoscopy and/or biopsy.
    In order to improve the accuracy of diagnosis and prevent the occurrence of esophageal cancer in the early phase, a quantitative analytical method used to discriminate among the various types of cancerous esophagus tissues is demanding to provide sufficient accuracy in cancer staging. Previous studies indicate that the structural change in collagen ascribes to misfolding of the triple helices that is highly associated with cancer cell invasion. Thus, it is of great significance to study the structural correlation between collagen fibers and esophageal cancer tissues at varying stages. Here we used polarization-resolved two-photon microscopy, not only the structural organization of collagen fibrils in squamous cell carcinoma was revealed at high spatial resolution but also the ratios of second harmonic generation (SHG) / two-photon excited fluorescence (TPEF), tensor elements of second-order nonlinear susceptibility, X22/X16, and SHG-circular dichroism (SHG-CD) were obtained.
        With the aid of the above results, we successfully classified esophageal cancers at four different stages, which complied well with the stage differentiation made by histopathology. This research indicated a reliable and highly accurate inspection of various types of esophageal cancer tissues, showing great potentials in cancer research.
    Advisory Committee
  • Fu-Jen Kao - chair
  • Ming-Che Chan - co-chair
  • Guan-Yu Zhuo - advisor
  • Cheng-Tang Pan - advisor
  • Files
  • etd-0623118-161304.pdf
  • Indicate in-campus at 2 year and off-campus access at 3 year.
    Date of Submission 2018-07-23

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