Title page for etd-0611118-123519


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URN etd-0611118-123519
Author Ya-Wen Hsu
Author's Email Address sponge668@gmail.com
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Department Institute of Biomedical Sciences
Year 2017
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Epithelial membrane protein 2 inhibits cell proliferation and induces apoptosis via TGFB-SP1 axis and recruitment of P2RX7 in urinary bladder urothelial carcinoma-derived cells
Date of Defense 2018-07-09
Page Count 72
Keyword
  • SP1 (Specificity protein-1)
  • TGFB
  • Epithelial membrane protein 2 (EMP2)
  • Urinary bladder urothelial carcinoma (UBUC)
  • Cell cycle arrest
  • Abstract In this study, our objective was to investigate the mechanisms of Epithelial membrane protein 2 (EMP2) inhibits cell proliferation and induces apoptosis in urinary bladder urothelial carcinoma-derived cells. A series of in vitro assays indicated that EMP2 regulates transforming growth factor beta 2 (TGFB2) and induces SMAD2/3 activation in bladder cancer cells. Herein, we show that signaling interplay between EMP2/TGFB and PI3K/AKT pathway in the regulation of several cyclin-dependent kinase inhibitors (CKIs). We showed that exogenous EMP2 expression upregulated CDKN1A, CDKN1B, CDKN1C and CDKN2B protein levels via the TGFB signaling pathway. Afterward, exogenous expression of the EMP2 gene upregulated CDKN1A, CDKN1B and CDKN1C protein levels through TGFB/SP1 activation in J82 cells. In addition, pull-down assay using anti-DDK antibody and immunoblotting with anti-P2RX7 antibody demonstrated that EMP2 interacted with the P2RX7 protein. Therefore, EMP2 induced both extrinsic and intrinsic apoptosis through interaction with P2RX7 protein in J82 cells. Combined, the current studies have identified EMP2 gene via TGFB/SP1 activation, and that involved in the regulation of cell cycle. Apart from this, our study indicate EMP2 inhibits cell proliferation and induces apoptosis through recruitment of P2RX7 protein.
    Advisory Committee
  • Chien-Feng Li - chair
  • Cheng-Tang Pan - co-chair
  • Hurng-Wern Huang - co-chair
  • Yow-Ling Shiue - advisor
  • Files
  • etd-0611118-123519.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2018-07-11

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