Title page for etd-0611117-143735


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URN etd-0611117-143735
Author Yang-kuang Pan
Author's Email Address No Public.
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Department Chemistry
Year 2016
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Molecular Imaging of Metabolites on Whole Body Skin by Ambient Mass Spectrometry
Date of Defense 2017-07-11
Page Count 104
Keyword
  • metabolites
  • skin
  • thermal desorption-electrospray ionization mass spectrometry
  • ambient mass spectrometry
  • molecular imaging
  • Abstract The adult skin provides the interface between the body and its outside world with a surface area of about 1.5 to 2 square meters. The role of the skin is to protect the human body from the outside world of various dangers. The chemical composition of the skin surface is still not well-defined. The traditional methods of detecting the surface composition of the skin is by gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS), which need for sample pretreatment and take a lot of time and manpower. In recent years, the development of atmospheric mass spectrometry (AMS) significantly reduces analytical times because it avoids complex sample pretreatment. This technique is also capble of analyzing the leaves of plants, tissue sections of various organs and microbial culture and could go even further in constructing relevant images. A new AMS technique, which is known as thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS), has the advantages of rapid, high-throughput analysis and non-invasive sampling. In this study, TD-ESI/MS was used as an analytical tool to find out the molecules present on the surface of human skin. We succeeded in scraping the skin with a probe to get the profile of the molecules on the skin, including caffeine and its metabolites, cholesterol, squalene and nicotine. Followed by a partition of the skin sampling position about 300 equally, and a sampling probe to obtain analyte from skin was putting into a heated oven for thermally desorbing analyte. Then, the analyte was sent to the Electrospray Ionization (ESI) region for post-ionizing and obtain the mass spectra. Finally, a specific molecular ion peak was captured from the mass spectrometry data for imaging. From the result of molecular imaging, the distribution of these molecules on the surface of the skin was found to be different, which in turn depended on the number or structure of glands under the skin. The molecular imaging by TD-ESI/MS will contributes to the identification and mapping of molecular distribution of various compounds on the surface of human skin, with promising potential applications in personal medicine in the coming future.
    Advisory Committee
  • Chien-Chen Lai - chair
  • Vinoth Kumar - co-chair
  • Jentaie Shiea - advisor
  • Files
  • etd-0611117-143735.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2017-07-11

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