Title page for etd-0603115-210823


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URN etd-0603115-210823
Author Ren-Tsung Wang
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2014
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Bactericidal mechanism of carboxyl group-modified bovine serum albumin and ovalbumin
Date of Defense 2015-06-30
Page Count 73
Keyword
  • Liposomes
  • Membrane-damaging activity
  • Bactericidal activity
  • Antimicrobial protein
  • Carboxyl groups modification
  • Abstract The aim of this study is to investigate the physicochemical properties and antimicrobial activities of semicarbazide-modified bovine serum albumin (BSA) and ovalbumin (OVA). MALDI-TOF and RP-HPLC showed that modification of carboxyl groups caused an increase in molecular weight and hydrophilicity of SEM-BSA and SEM-OVA compared with those of BSA and OVA. CD spectra measurement revealed that modification of carboxyl groups caused a change in the secondary structure of BSA and OVA. SEM-BSA exhibited a growth inhibition on E. coli and S. aureus, while SEM-OVA only shows bactericidal activity against S. aureus. Destabilization of structural stability of lipopolysaccharide (LPS) or inhibition of lipoteichoic acid (LTA) synthesis promoted antibacterial activity of SEM-BSA and SEM-OVA. Propidium iodide (PI) staining indicated that SEM-BSA and SEM-OVA induced membrane permeability of S. aureus. Compared to SEM-BSA, SEM-OVA insignificantly affected the membrane permeability of E. coli. SEM-BSA and SEM-OVA induced membrane fusion and permeability of S. aureus membrane-mimicking vesicles. Unlike SEM-BSA, SEM-OVA did not damage E. coli membrane-mimicking vesicles. Both LPS and LTA suppressed membrane-damaging activity of SEM-BSA and SEM-OVA. SEM-BSA showed higher binding affinity with bacterial membrane-mimicking vesicles compare to SEM-OVA. Moreover, SEM-BSA penetrated into membrane and perturbed the membrane structure. The interacted-mode of SEM-OVA with S. aureus and E. coli membrane-mimicking vesicle differed. Taken together, the antibacterial action of SEM-BSA and SEM-OVA are related to their ability to damage bacterial membrane. The membrane-interacted mode of SEM-OVA may elucidate its inability to display antibacterial activity against E. coli.
    Advisory Committee
  • Chun-chang Chang - chair
  • Shinne-Ren Lin - co-chair
  • Long-Sen Chang - advisor
  • Files
  • etd-0603115-210823.pdf
  • Indicate in-campus at 3 year and off-campus access at 3 year.
    Date of Submission 2015-07-07

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