Title page for etd-0601113-130017


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URN etd-0601113-130017
Author Hsin-I Chien
Author's Email Address No Public.
Statistics This thesis had been viewed 5386 times. Download 192 times.
Department Biological Sciences
Year 2012
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Molecular typing of Methicillin-Resistant Staphylococcus aureus specimens from Southern Taiwan
Date of Defense 2013-05-29
Page Count 43
Keyword
  • multiplex polymerase chain reaction
  • multiple-drug resistant
  • HA-MRSA
  • CA-MRSA
  • Panton-Valentine leukocidin
  • Abstract A majority of the methicillin-resistant Staphylococcus aureus (MRSA) was thought as
    Healthcare-associated MRSA (HA-MRSA), which has been recognized as main
    causative agent of nosocomial infection. Contrarily, the community-associated MRSA
    (CA-MRSA) was reported that frequently isolated from skin and soft tissues in a
    hospital environment. In addition, these MRSA isolates usually expressed PVL gene
    (Panton-valentine leukocidin, PVL). In this study, we have collected 320 bacterial
    isolates comprised of pre-identified MRSA isolates from the specimens of patients in
    Kaohsiung Armed Forces General Hospital during 2007 to 2010. For bacterial
    identification, in addition to routinely culture-based method, the antibiotics
    susceptibility test was performed, and multiplex polymerase chain reaction (mt-PCR)
    was implemented for SCCmec typing and each observed spa type was confirmed by
    DNA direct sequencing. In this study, we investigated the drug resistance of those
    collected MRSA isolates by which genotyping of SCCmec as well as detecting on
    whether presecnce of mecA and PVL. Moreover, the clinical impact of whether
    intergretiing molecular data and bacterial epidemiological information can successfully
    distinguishs the CAMRSA from HA-MRSA and predicts nosocomial infection in our
    hospital was evaluated. As a result, we found a majority of the MRSA isolates were
    SCCmec IV or V (66.6%) with multiple-drug resistance (MDR). In summry, we utilize
    mt-PCR technique for SCCmec genotyping and to establish the molecular database of
    MRSA strains in this study. Our results might provide a quick reference for decision
    making of antimicrobial therapy.
    Advisory Committee
  • Jong-Kang Liu - chair
  • Ming-Der Shi - co-chair
  • Jiin-Tsuey Cheng - advisor
  • Files
  • etd-0601113-130017.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2013-07-03

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