| URN |
etd-0529116-155910 |
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| Author |
Jing-fong Liao |
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| Author's Email Address |
a790207@hotmail.com |
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| Statistics |
This thesis had been viewed 5571 times. Download 1255 times. |
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| Department |
Institute of Medical Science and Technology |
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| Year |
2015 |
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| Semester |
2 |
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| Degree |
Master |
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| Type of Document |
|
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| Language |
zh-TW.Big5 Chinese |
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| Title |
Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine |
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| Date of Defense |
2016-06-18 |
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| Page Count |
97 |
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| Keyword |
Transcutaneous immunization
Dissolving microneedle
DNA vaccine
Flexible patches
Polyvinyl alcohol
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| Abstract |
This study developed a flexible and rapidly-dissolvable microneedle (MN) patch for transdermal delivery and stability enhancement of DNA vaccine. The MN patch is made by polyvinyl alcohol (PVA) with the properties of rapid dissolution, biocompatibility and film-forming. Besides, the vaccine encapsulated in MN would be rapidly released after insertion of the MN patch to the skin. We were able to encapsulate 22.4 μg of PCV2 plasmid DNA vaccine in MNs per patch with a loading rate of 87.8 wt % when casting the PCV2 plasmid DNA vaccination the PDMS mode surface. We also done the stability study, the activity of vaccine encapsulated in MNs can be maintained exceed 111 days without any damage at 37 ℃ of storage. The ex vivo study demonstrated that the MNs have excellent mechanical strength to puncture the stratum corneum and needles can be dissolved within 10 minutes to release the vaccine in epidermis then the hole can be quickly healed within 40 minutes to avoid the infection risk. The Animals studies showed that the induced antibody titers via MN patch administration were about 10-time higher than intramuscular (IM) administration after 3 weeks of vaccination. The results confirmed that the MNs can induce high antibody concentration in body to induce the strongest immune responses after immunization. |
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| Advisory Committee |
Chen-Chi M. Ma - chair
Jin-Ching Lee - co-chair
Hung-Wei Yang - advisor
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| Files |
Indicate in-campus at 1 year and off-campus access at 2 year. |
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| Date of Submission |
2016-07-25 |
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