Title page for etd-0529115-094154


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URN etd-0529115-094154
Author Yen-fu Lin
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2014
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The Studies on Crystal Stability of Virus-like Particle from Dragon Grouper Betanodavirus
Date of Defense 2015-06-09
Page Count 183
Keyword
  • virus-like particles
  • Dragon grouper nervous necrosis virus
  • crystallization
  • cross-linking
  • Abstract Dragon grouper nervous necrosis virus (DGNNV), a betanodavirus, is the cause of viral nervous necrosis in dragon grouper (Epinephelus lanceolatus). In larvae and juveniles, mortality is up amount 100%. The capsid protein of the DGNNV form virus-like particles (VLPs) that were expressed in Escherichia coli. So far, Crystals of VLPs diffracted X-rays to ~7.5 Å resolution (Luo and Lin, 2014). In this study, VLPs purified in phosphate buffer were cross-linked with DSS grown to crystals of higher resolution of diffracted X-rays. Among poly-His tagged VLPs and point-mutation, C338C6H can not form crystals while N25R29A mutant resulted in bigger size. VLPs for studies of DGNNV VLPs protein structure and function.
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Liu, Wangta - co-chair
  • Hong Yi-Ren - co-chair
  • Min-Ying Wang - co-chair
  • Chain-Shing Lin - advisor
  • Files
  • etd-0529115-094154.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2015-06-29

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