Title page for etd-0521115-005317


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URN etd-0521115-005317
Author Jenn-tzong Chang
Author's Email Address No Public.
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Department Biological Sciences
Year 2014
Semester 2
Degree Ph.D.
Type of Document
Language English
Title Genome and Infection Characteristics of Human Parechovirus Type 1
Date of Defense 2015-06-12
Page Count 71
Keyword
  • type I interferon
  • parechovirus
  • HPeV
  • innate immunity
  • Abstract Human parechoviruses (HPeVs), members of the family Picornaviridae, are associated with severe human clinical conditions such as gastrointestinal disease, encephalitis, meningitis, respiratory disease and neonatal sepsis. A new contemporary strain of HPeV1, KVP6 (accession no. KC769584), was isolated from a clinical specimen. Full-genome alignment revealed that HPeV1 KVP6 shares high genome homology with the German strain of HPeV1, 7555312 (accession no. FM178558) and could be classified in the clade 1B group. An intertypic recombination was shown within the P2-P3 genome regions of HPeV1. Cell-type tropism test showed that T84 cells (colon carcinoma cells), A549 cells (lung carcinoma cells) and DBTRG-5MG cells (glioblastoma cells) were susceptible to HPeV1 infection, which might be relevant clinically. A facilitated cytopathic effect and increased viral titers were reached after serial viral passages in Vero cells, with viral genome mutation found in later passages. HPeV1 is sensitive to elevated temperature because 39C incubation impaired virion production. HPeV1 induced innate immunity with phosphorylation of interferon (IFN) regulatory transcription factor 3 and production of type I IFN in A549 but not T84 cells. Furthermore, type I IFN inhibited HPeV1 production in A549 cells but not T84 cells; T84 cells may be less responsive to type I IFN stimulation. Moreover, HPeV1-infected cells showed downregulated type I IFN activation, which indicated a type I IFN evasion mechanism. The characterization of the complete genome and infection features of HPeV1 provide comprehensive information about this newly isolated HPeV1 for further diagnosis, prevention or treatment strategies.
    Advisory Committee
  • Jiin-Tsuey Cheng - chair
  • Chung-Lung Cho - co-chair
  • Yao-Shen Chen - co-chair
  • Tsung-Hsien Chang - advisor
  • David Chao - advisor
  • Files
  • etd-0521115-005317.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2015-06-22

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