Title page for etd-0513117-121727


[Back to Results | New Search]

URN etd-0513117-121727
Author CHIA-YU LIN
Author's Email Address 4A0H0049@stust.edu.tw
Statistics This thesis had been viewed 5357 times. Download 0 times.
Department Marine Biotechnology and Resources
Year 2016
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The Study on the Shell-Domain Structure of Capsid Protein from Dragon Grouper Betanodavirus
Date of Defense 2017-05-26
Page Count 142
Keyword
  • capsid protein
  • virus-like particles
  • crystallization
  • Dragon grouper nervous necrosis virus
  • Abstract Nodaviridae is divided into Alphanodavirus and Betanodavirus. Alphanodavirus and Betanodavirus infect insects and fish, respectively. The infection of Betanodavirus have been demonstrated the cause of nerve necrosis in several fishes. For instance, the nervous necrosis in dragon grouper (Epinephelus lanceolatus) was infected by dragon grouper nevous necrosis virus (DGNNV) that is a member of genus Betanodavirus. The infection of DGNNV was shown that it resulted in abnormal movement and darkness in body color, with mortality as high as 100%. The capsid proteins of the DGNNV assembled into virus-like particles (VLPs) and that were expressed in Escherichia coli. In this study, VLPs were purified in phosphate buffer and Tris buffer. The VLPs of DGNNV were further treated with NTCB、CNBr. The structure information of the Shell-Domain Structure of Capsid Protein from Dragon Grouper was observed and determined by electron microscope (JEOL JEM-2100) as well as X-ray diffraction. In addition, how the effects of glycerol, antibodies and metal ions on crystallization were also investigated. Finally, a variety of 3D prediction softwares were used to predict of the structure of DGNNV and then the genetic relationship among nevous necrosis viruses was analyized and compared.
    Advisory Committee
  • Chiu-Hao Chen - chair
  • Wangta Liu - co-chair
  • Min-Ying Wang - co-chair
  • Chan-Shing Lin - advisor
  • Files
  • etd-0513117-121727.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2017-06-15

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys