Title page for etd-0513116-115112


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URN etd-0513116-115112
Author Po-han Chu
Author's Email Address No Public.
Statistics This thesis had been viewed 5384 times. Download 519 times.
Department Biological Sciences
Year 2015
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Using PCP and dioxin to induce dioxin-degrading genes expression in Pseudomonas mendocina NSYSU
Date of Defense 2016-06-03
Page Count 237
Keyword
  • pentachlorophenol
  • P. mendocina NSYSU
  • dioxin
  • qPCR
  • degrading genes
  • Abstract Pseudomonas mendocina strain NSYSU was isolated from a dioxin-contaminated soil in 2002. Previous studies showed that this strain could degrade various dioxins under aerobic and anaerobic conditions. We also found the efficiency of dioxin-degrading could be enhanced after pentachlorophenol (PCP) inducing. Preliminary studies showed that P. mendocina NSYSU might contain nine dioxin-degrading genes including dechlorination and ring-cleavage genes. Therefore in this study, we used real-time PCR to detect the expression of these dioxin-degrading genes after inducing by PCP, OCDD, and OCDF. The results showed P. mendocina NSYSU contains two ring-cleavage genes (Pmen_0474, Pmen_2526) and five dechlorination genes (Pmen_0392, Pmen_1621, Pmen_3718, Pmen_4219, Pmen_4457). The PCP induction study revealed that the expression of these 7 dioxin-degrading genes boosted 5.58 folds at 0.5~5.5 hours after inducing by 40 ppm PCP. The OCDD induction study showed that the expression of these 7 dioxin-degrading genes increased 3.69 folds at 10~14.5 hours after inducing by 0.1 ppm OCDD. For the OCDF induction study, the results showed that the expression of these 7 dioxin-degrading genes slightly increased 1.79 folds at 11~15 hours after inducing by 0.1 ppm OCDF. In summary, the expression of all these 7 dioxin-degrading genes was successfully induced by PCP, OCDD and OCDF under aerobic conditions, in which PCP performed the best induction effect.
    Advisory Committee
  • Jimmy C. M. Kao - chair
  • Wei-Chih Lin - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0513116-115112.pdf
  • Indicate in-campus at 1 year and off-campus access at 1 year.
    Date of Submission 2016-06-16

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