Title page for etd-0328111-200359


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URN etd-0328111-200359
Author Hsiao-ying Lee
Author's Email Address No Public.
Statistics This thesis had been viewed 5574 times. Download 1272 times.
Department Biological Sciences
Year 2010
Semester 2
Degree Master
Type of Document
Language English
Title Effect of the m-3M3FBS on Ca2+ movement in human SCM1 gastric cancer cells
Date of Defense 2010-12-10
Page Count 36
Keyword
  • Ca2+
  • m-3M3FBS
  • SCM1
  • human gastric cancer cells
  • Abstract m-3M3FBS is a new compound that has been used as a phospholipase C (PLC)
    activator. The effect of m-3M3FBS on cytosolic free Ca2+ concentrations in human
    gastric cancer cells (SCM1) is unclear. This study explored whether m-3M3FBS
    changed basal [Ca2+]i levels in suspended SCM1 cells by using fura-2 as a
    Ca2+-sensitive fluorescent dye. m-3M3FBS at concentrations between 1-50 μM
    increased [Ca2+]i in a concentration-dependent manner. The Ca2+ signal was reduced
    partly by removing extracellular Ca2+. This Ca2+ influx was inhibited by phospholiapase
    A2 inhibitor aristolochic acid , store-operated Ca2+ channel blockers nifedipine 、
    econazole and SK&F96365; and protein kinase C inhibitor GF109203X. Phorbol
    12-myristate 13-acetate ([PMA] a protein kinase C activator) had no effect on
    m-3M3FBS-induced [Ca2+]i rise. In Ca2+-free medium , pretreatment with m-3M3FBS
    abolished thapsigargin (TG) or 2,5-di-tert-butylhydroquinone (BHQ) - induced [Ca2+]i
    rise. Conversely, pretreatment with the endoplasmic reticulum Ca2+ pump inhibitors TG
    or BHQ partly inhibited m-3M3FBS -induced Ca2+ release. The inhibition of PLC with
    U73122 did not alter mMIRC. Collectively, in SCM1 cells, mMIRC by causing PLCindependent
    Ca2+ release from the endoplasmic reticulum and Ca2+ influx via
    phospholipase A2-protein kinase C-sensitive store-operated Ca2+ channels.
    Advisory Committee
  • Chen-Chih Kao - chair
  • Ko-L Lin - co-chair
  • Chung-Ren Jan - advisor
  • Shaw, Chen-Fu - advisor
  • Files
  • etd-0328111-200359.pdf
  • indicate access worldwide
    Date of Submission 2011-03-28

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