Title page for etd-0314116-101517


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URN etd-0314116-101517
Author Ting-Hsuan LIn
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2015
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Antifouling activity of natural compounds and their derivatives against barnacle (Amphibalanus amphitrite) cypris larvae
Date of Defense 2016-05-31
Page Count 115
Keyword
  • antifouling activity
  • cypris larvae
  • fouling organism
  • barnacle
  • natural compounds
  • Abstract Biofouling is a serious global issue of our oceans. Marine fouling organisms can attach on immersed artifical surfaces thus thus cause economic loss. This study utilized the major fouling barnacle species Amphibalanus amphitrite to test anti-fouling activities of more than one hundred natural compounds, including crude extracts, pure compounds and the derivatives isolated from marine organisms. There are more than one hundred natural compounds isolated from marine organisms and the derivatives were screened. There are three steps in our experiments: (1) Preliminary test: we prepared all natural compounds and the derivatives at the concertation of 10 ppm or 20 ppm and recorded the settlement condition of barnacle cypris larvae for 48 hours. (2) Antifouling activity test: we tested antifouling activities of potential compounds from step 1 at concentrations from 1-100 ppm. Then we estimated half maximal effective concentration (EC50), half lethal concentration (LC50) and therapeutic ratio (TR) of each compound. (3) Field test: we mixed the candidate compound with marine paint, then observed actual effect of the antifouling paint in the field. Our results showed that the pure compound S28 has high antifouling activity. The EC50 of S28 is 8.824 ppm, LC50 is 101.080 ppm and the therapeutic ratio (TR) is 11.454. These results suggested S28 is effective and has low toxicity. In conclusion, S28 has great potential for future development of antifouling paint.
    Advisory Committee
  • Kuo-Chin Chen - chair
  • Chih-Chuang Liaw - co-chair
  • Hsiu-Chin Lin - advisor
  • Files
  • etd-0314116-101517.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2016-06-24

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