Title page for etd-0204109-012344


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URN etd-0204109-012344
Author Li-ni Kuo
Author's Email Address No Public.
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Department Biological Sciences
Year 2008
Semester 1
Degree Master
Type of Document
Language English
Title Effect of thimerosal on Ca2+ movement and viability in
human oral cancer cells
Date of Defense 2009-01-15
Page Count 55
Keyword
  • oral cells
  • OC2
  • apoptosis
  • Ca2+
  • thimerosal
  • Abstract The effect of thimerosal on cytosolic free Ca2+ concentrations ([Ca2+]i) in human oral cancer cells (OC2) is unclear. This study explored whether thimerosal changed basal [Ca2+]i levels in suspended OC2 cells by using fura-2. Thimerosal at concentrations between 1-50μM increased [Ca2+]i in a concentration-dependent manner. The Ca2+ signal was reduced partly by removing extracellular Ca2+. Thimerosal-induced Ca2+ influx was not blocked by L-type Ca2+ entry inhibitors and protein kinase C modulators [phorbol 12-myristate 13-acetate (PMA) and GF109203X]. In Ca2+-free medium, 50μM thimerosal failed to induce a [Ca2+]i rise after pretreatment with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). Inhibition of phospholipase C with U73122 did not change thimerosal-induced [Ca2+]i rises. At concentrations between 5 and 10μM thimerosal killed cells in a concentration-dependent manner. The cytotoxic effect of 8μM thimerosal was potentiated by prechelating cytosolic Ca2+ with the Ca2+ chelator BAPTA/AM. Flow cytometry data suggested that 1-7μM thimerosal induced apoptosis in a concentration-dependent manner. Collectively, in OC2 cells, thimerosal induced [Ca2+]i rises by causing phospholipase C-independent Ca2+ release from the endoplasmic reticulum and Ca2+ influx via non-L-type Ca2+ channels. Thimerosal killed cells in a concentration-dependent manner via apoptosis.
    Advisory Committee
  • Chen-Chih Kao - chair
  • Chung-Ren Jan - advisor
  • Chen-Fu Thiao - advisor
  • Files
  • etd-0204109-012344.pdf
  • indicate access worldwide
    Date of Submission 2009-02-04

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