Title page for etd-0120117-104748


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URN etd-0120117-104748
Author Zhi-Cheng Chen
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2016
Semester 2
Degree Ph.D.
Type of Document
Language zh-TW.Big5 Chinese
Title The effects of skeleton extract obtained from marine organisms on wound healing
Date of Defense 2017-01-12
Page Count 141
Keyword
  • wound healing
  • Shijueming
  • os sepiae
  • macrophage
  • transforming growth factor-beta
  • Abstract Shijueming (SJM) is a traditional Chinese medicine. Records from 1757, in the Qing dynasty, detail the use of SJM for treating skin injuries, particularly poorly managed ulcers or traumatic wounds. However, no study has yet adopted an animal model to verify the phenomenon described in these records regarding the efficacy of SJM in promoting wound healing. In studies identifying novel bioactive materials in natural products that are useful for treating skin injuries, os sepiae (OS) extract has been reported to have a wound-healing effect. Therefore, this study used in vitro and in vivo models and performed tissue section analysis and Western blotting to evaluate the effect of SJM and OS on wound healing. RAW264.7 cells were used in antiinflammatory activity and phagocytic assays. Male Wistar rats were used to evaluate the effect of SJM and OS on burn and excision wound healing. The results were analyzed through hematoxylin and eosin staining, picrosirius red staining, and Western blotting. The results revealed that in the in vitro model, SJM promoted the phagocytic activity of RAW264.7 cells and OS promoted EA. hy 926 endothelial cell migration. Both SJM and OS reduced inducible nitric oxide synthase (iNOS) expression and enhanced macrophage phagocytosis in vitro. Compared with vehicle-treated rats, both SJM and OS significantly promoted wound healing in the rat burn injury model. Furthermore, SJM reduced neutrophil infiltration and promoted transforming growth factor-beta 1 (TGF-β1) protein expression, thus increasing the collagen I content. We suggest that the mechanism through which SJM and OS promote wound healing is related to macrophage activation and angiogenesis enhancement, respectively. In the inflammatory phase, both SJM and OS alleviate inflammation by inhibiting iNOS expression and removing neutrophils through phagocytosis. Furthermore, SJM induces TGF-β1 secretion, converting collagen during the tissue remodeling phase. These findings indicate that SJM is a promising therapeutic option for treating burn injury.
    Advisory Committee
  • Ming-Hong Tai - chair
  • Wen-Sheng Liu - co-chair
  • Hsiu-Chin Lin - co-chair
  • Hui-Min Wang - co-chair
  • Chien-Chih Chiu - co-chair
  • Yao-Chang Chen - co-chair
  • Zhi-Hong Wen - advisor
  • Files
  • etd-0120117-104748.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2017-02-20

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