Title page for etd-0116115-105446


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URN etd-0116115-105446
Author Ching-I Chen
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2014
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Analyses of the Bacterial Communities Surrounding Dongsha Island by Next Generation Sequencing
Date of Defense 2015-02-11
Page Count 154
Keyword
  • PCR
  • Dongsha atoll national park
  • DGGE
  • microbial diversity
  • next generation sequencing
  • Abstract Microbacteria are everywhere even they can’t been seen by eyes directly. There are seventy-one percent of the earth’s surface covered by ocean. Within different location or ocean current etc. causing intense different environment. Marine bacteria have been through evolution all the time begin. We can discover the existence of bacterial no matter at the deep-sea with high pressure, or volcano on the sea-bed. There will be development different bacterial community structure according to the environmental factors. So we can analysis the relationship between the area and bacterial diversity by collecting the sample in different location. In this study, we sampled the surface seawater and the sediment from Dongsha island to research the bacterial community structure. But due to 99% of marine bacteria can not be incubated in laboratory, we use high throughput sequencing technique to analysis uncultured marine bacteria. Dongsha island doesn’t open to the public, so we infer the microbiota may be more close to the origin environment. Pass through the sequencing analysis we found that the Gammaproteobacteria is the dominant class not only in the sediment but also in seawater. But the most common dominant bacterium in the normal sea water is Alphaproteobacteria not Gammaproteobacteria. We infer that Dongsha is a island in rich of coral reef and the Gammaproteobacteria is one of the dominant bacterium associate with coral or they were been washed away from land in to aqua environment. So we can find abundance Gammaproteobacteria in our sample no matter in sediment or sea water. The subdominant bacterium is Alphaproteobacteria in seawater and Bacilli in sediment. Maybe it is come from the organic product of plant on the land by wade.
    Advisory Committee
  • Chan-Shing Lin - chair
  • Liu , Jong-Kang - co-chair
  • Chi-Hsin Hsu - co-chair
  • Shu-Fen Chiou - advisor
  • Files
  • etd-0116115-105446.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2015-02-16

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