Title page for etd-0115116-075905


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URN etd-0115116-075905
Author Chun-feng Yang
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2015
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The disturbance effect of phthalate DEHP on camptothecin-treated human breast cancer cells in vitro and in zebrafish xenograft model
Date of Defense 2016-01-15
Page Count 83
Keyword
  • DNA double-strand breaks
  • camptothecin
  • Phthalate
  • Bis(2-ethylhexyl) phthalate
  • DEHP
  • zebrafish
  • chemoresistance
  • breast cancer
  • Abstract Breast cancer is a leading cause of death in women worldwide, and chemotherapy is one of the primary strategies for breast cancer treatment. However, the chemoresistance often causes the poor prognosis of breast cancer patients. Phthalate derivatives have been shown to affect the expression of genes, and could take effects on genotoxic sensitivity and the ability of DNA repair in cells. Recent evidence suggested that phthalate exposure may be closely correlated with the chemoresistance of breast cancer cells. However, the underlying mechanism that phthalate causes chemoresistance is largely unclear. In the study, we investigated whether phthalates affect the chemoresistance by reinforcing the ability of DNA repair. Our results showed that phthalate Bis(2-ethylhexyl) phthalate (DEHP) attenuates the camptothecin (CPT)-induced DNA damage in breast cancer cells MCF-7. Moreover, phthalate DEHP pre-treatment significantly inhibits the two hallmarks of double-strand breaks (DSB) of DNA, including γH2AX and the phosphorylation of ATM, which were significantly reduced in CPT-treated MCF-7 cells. Consistently, the results of zebrafish xenograft assay confirmed the protective effect of DEHP on CPT-induced cytotoxicity of breast cancer. Accordingly, our present work suggests that phthalate DEHP may affect the anti-breast cancer effect of CPT through attenuating the induction of DNA damage.
    Advisory Committee
  • Jeff Yi-Fu Chen - chair
  • Kuang-hung Cheng - co-chair
  • Chien-Chih Chiu - advisor
  • Hung-Wen Huamg - advisor
  • Files
  • etd-0115116-075905.pdf
  • Indicate in-campus at 3 year and off-campus access at 3 year.
    Date of Submission 2016-02-15

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