Title page for etd-0113111-173303


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URN etd-0113111-173303
Author Te-fang Chao
Author's Email Address No Public.
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Department Biological Sciences
Year 2010
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Enhancement of growth and migration of human breast cancer cell (MDA-435S) by human C1 inhibitor
Date of Defense 2010-07-16
Page Count 84
Keyword
  • breast cancer cell
  • growth and migration
  • human C1 inhibitor
  • Abstract C1-esterase inhibitor (C1-inh) can inhibitor the first complement protein as C1s and C1r activity to reach adjust classical pathway, avoid excessive activation of the complement system to cause disease. C1-inhibitor protein composed of 478 amino acids with two domains: C terminal domain (serpin domain) and N terminal domain. The early focus has been to angioedema associated with cancer found so far. So the purpose of the study was to investigate whether the C1-inh cause for the breast cancer cell proliferation and migration. We use recombinant gene transform Escherichia coli strain BL21(DE3) and expression. Recombinant protein was purified using affinity column. Influence of proliferation and migration on breast cancer cells were tested by purified recombinant C1-inh. In breast cancer cell proliferation results showed, C1-inh significant proliferation of breast cancer, and when the higher concentration, the longer the incubation time, the remarkable effect of promoting proliferation is even more obvious. The results in breast cancer cell migration is also significant in the C1-inh to promote breast cancer cell migration, and when the higher concentration of the longer incubation time, the significant increased migration is more effective. Therefore, this study does note C1-inh to promote breast cancer cell proliferation and migration.
    Advisory Committee
  • Hung-Tu Huang - chair
  • Hsiu-Moan Kuo - co-chair
  • Shi-ping He - advisor
  • Files
  • etd-0113111-173303.pdf
  • indicate accessible in a year
    Date of Submission 2011-01-13

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