Title page for etd-0111114-133314


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URN etd-0111114-133314
Author Chien-ming Chen
Author's Email Address No Public.
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Department Biological Sciences
Year 2013
Semester 1
Degree Ph.D.
Type of Document
Language zh-TW.Big5 Chinese
Title A study of OSTC/DC2, a novel subunit of oligosaccharyltransferase, after heat shock
Date of Defense 2014-01-22
Page Count 177
Keyword
  • oligosaccharyltransferase
  • polyclonal antibody
  • gene knockdown
  • heat shock
  • glycosylation
  • glycoprotein
  • Abstract OSTC, a Homo sapiens OSTC/DC2 protein, belongs to a new subunit of mammalian oligosaccharyltransferase (OST). OST transfers the high-mannose sugars to nascent polypeptides during N-linked glycosylation in the lumen of the rough endoplasmic reticulum. In this study, we used synthetic peptides (RMKLPGYLMG) as an antigen to develop a polyclonal antibody against OSTC/DC2. After heat treatment of Balb/c mice, OSTC/DC2 showed up-regulated in some tissues of brain, and peaked at the 12th hour of recover stage. Furthermore, OSTC/DC2 was also up-regulated in cell lines during heat-shock treatment. The strikingly immediate up-regulation of OSTC/DC2 in response to heat shock is only seen in the cortex, suggesting that OSTC/DC2 may have unique functions in neurons. In addition, OSTC/DC2 induction is synchronous to increased expression of HSP70 and activation of AKT after heat shock, supporting the notion that OSTC/DC2 may play a significant role in heat shock response.
    Advisory Committee
  • Chao, David - chair
  • Liu, Jong-Kang - co-chair
  • Liu, Wangta - co-chair
  • Tseng Chao-Neng - co-chair
  • Cho, Chung-Lung - advisor
  • Files
  • etd-0111114-133314.pdf
  • indicate access worldwide
    Date of Submission 2014-02-11

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