Title page for etd-0026117-100411


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URN etd-0026117-100411
Author Cheng-jou Tan
Author's Email Address No Public.
Statistics This thesis had been viewed 5357 times. Download 207 times.
Department Biological Sciences
Year 2016
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Population structure of Formosan Reeves‘ muntjac (Muntiacus reevesi micrurus) and genetic diversity in isolated habitat, based on Mitochondrial DNA
Date of Defense 2017-01-13
Page Count 73
Keyword
  • Taiwan
  • cytochrome b
  • Shoushan National Nature Park
  • Muntiacus reevesi micrurus
  • D-loop
  • Abstract The Formosan Reeves' muntjac (Muntiacus reevesi micrurus) is the smallest Cervidae species in Taiwan. It inhabits from plain to mountains at elevation of 3000 meters in Taiwan. Some of M. micrurus populations are found in isolated areas such as Shoushan National Natural Park. So far, only one study investigated the genetic differentiation of M. micrurus among 13 areas sampled island-wide from Taiwan, based on partial D-loop sequence. The results showed no significant genetic differentiation among these areas. However, no samples from isolated areas were included in the study. The Shoushan National Nature Park is located in Kaohsiung, isolated from Central Mountain Range by human development. This study aimed to use whole cytochrome b and D-loop sequences to analyze the genetic structure of M. micrurus populations from Taiwan, and to study the M. micrurus genetic differentiation in the isolated habitat in Shoushan Natural Nature Park. DNA samples of M. micrurus where collected from 8 regions over Taiwan. Thirty-one haplotypes were identified from the 151 sequenced samples. The sequence divergences in cytochrome b and D-loop sequence were 0.6% and 3.8%, respectively. The haplotype divergences were highest between samples from northen and southen Taiwan, and almost all haplotypes were endemic to the region where they were sampled, except two of haplotype found in three regions. As the overall a mean haplotype diversity was higher than 0.5 and the nucleotide diversity lower than 0.005. The populations of M. micrurus in Taiwan probably had undergone the low effect population size. The haplotype diversity in Shoushan National Nature Park was low, only higher than Fushan Botanical Garden and its nucleotide diversity was higher than that of Fushan Botanical Garden, Taroko National Park and Dan Da. The results of AMOVA indicated the genetic differentiation were most significant(CT = 0.5778,P < 0.001)when samples from Taroko National Park and Dan Da were combined along with the other 6 to form 7 groups. IBD analysis showed the genetic differentiation between populations increased with distance. The phylogeographic structure suggested that haplotypes from Taroko National Park and Shei Pa National Park ancient haplotype and have been in radial expansion. The five haplotypes identified from Shoushan National Nature Park were distributed in the periphery of the phylogeographic structure. M. micrurus in Taiwan might originally have moved then dispersed from the north to the south along the land bridge formed between Taiwan and China in the glacier age. Later, the geographic differentiations among these regions decreased due to gene flow between regions and resulted with genetic differentiation between populations in the north and south Taiwan. The low genetic diversity of in the population from Shoushan National Nature Park is probably the result of its geographic isolation and restricted gene flow from other regions.
    Advisory Committee
  • Jai-Chyi Pei - chair
  • Yu-Ten Ju - co-chair
  • Hsueh-Wen Chang - advisor
  • Files
  • etd-0026117-100411.pdf
  • Indicate in-campus at 1 year and off-campus access at 1 year.
    Date of Submission 2017-02-06

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