Title page for etd-0020115-161552


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URN etd-0020115-161552
Author Chiu-chin Lu
Author's Email Address No Public.
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Department Department of Oceanography
Year 2014
Semester 1
Degree Ph.D.
Type of Document
Language zh-TW.Big5 Chinese
Title An extracellular short-chain peptide ABMOK1 from the benthic diatom Nitzschia sp. and its antimicrobial activity
Date of Defense 2015-01-16
Page Count 110
Keyword
  • LC-MS-MS
  • SEM
  • Nitzschia sp.
  • Antimicrobial peptides
  • Marine benthic diatoms
  • Abstract Most of antimicrobial peptides (AMPs) are less than 10 kDa in molecular weight, hydrophobic, membrane active, and have an overall net positive charge. To date, few AMPs have been isolated from marine diatoms. This research studied novel AMPs from marine benthic diatoms and examined their antimicrobial activity. Extracellular extracts of the benthic diatom Nitzschia sp. 1 CCL-2013 were analyzed; the major fraction was less than 6.5 kDa of molecular weight. LC-MS/MS analysis on this major fraction identified 336 peptides. Out of the 336 peptides, 6 potential AMPs were identified using the following strigent critera: being 10 to 15 aa in length, having more than positive one to three in total net charge and at percentage of hydrophobic residues between 0.53 and 0.6. One peptide which was named as ABMOK1 was arbitrarily picked from the 6 peptides and tested for its anbitiotic activity. This novel AMP, ABMOK1, is characterized by the sequence of CGYCGACVGVCK. The SEM of images of Escherichia coli treated by 100 g/mL of ABMOK1 showed that holes were punched on the bacterial membrane, suggesting that ABMOK1 is a membrane-active antibacterial peptide which takes a detergent-like mode of destructive action.
    Advisory Committee
  • Tse-Min Lee - chair
  • Hsueh-Wei Chang - co-chair
  • Long-Sen Chang - co-chair
  • Hung-Wen Huamg - co-chair
  • Hin-Kiu Mok - advisor
  • Houng-Yung Chen. - advisor
  • Files
  • etd-0020115-161552.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2015-01-20

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