Title page for etd-0019116-144920


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URN etd-0019116-144920
Author Ming-Ju Tsai
Author's Email Address No Public.
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Department Mechanical and Electro-Mechanical Engineering
Year 2015
Semester 1
Degree Master
Type of Document
Language English
Title Stretching of DNA Molecules and Flow Field Characteristic in Semi-Circle and Quartered-Circle Microchannels.
Date of Defense 2016-01-13
Page Count 201
Keyword
  • μPIV
  • DNA molecules
  • hydrodynamic stretch
  • electroosmotic driven
  • shear flow
  • Abstract In this study, used electroosmotic-driven to generate shear flow in semi-circle and quartered-circle microchannel which were designed to investigate the stretching of λDNA molecules and flow phenomena. We utilized μPIV to measure the velocity distribution and FM to observe the stretching of dyed λDNA in microchannel. This study, we analyzed statistically stretch ratio and relaxation time of λDNA molecules in 1×TBE buffer solution with a variety of viscosity and intensity of electric field. Then the stretching force, shear rate, and spring constant were calculated and compared with conventional model. It was found that higher Wi and Pe can have longer stretch length, and smaller curvature of radius in the semi-circle microchannel could make better stretching consequent.
    Advisory Committee
  • Ching-Jeng Ho - chair
  • Chao-Kuang Chen - co-chair
  • Shou-Shing Hsieh - advisor
  • Files
  • etd-0019116-144920.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2016-01-20

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