Title page for etd-0003118-150231


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URN etd-0003118-150231
Author Ying-ting Wang
Author's Email Address No Public.
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Department Chemistry
Year 2017
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules
Date of Defense 2017-12-15
Page Count 72
Keyword
  • fluorescence imaging
  • urea
  • hemoglobin
  • pH
  • copper nanoclusters
  • Abstract In recent years, noble metal nanoclusters have been extensively studied, while the research on copper nanoclusters is relatively rare. Gold, silver, and copper are contained in the periodic table of the same group of elements. Copper is Earth-abundant, low prices and relatively friendly to humans. In this study, we direct use of pepsin as a template, so that with high biocompatibility and without additional modification. And we also use low toxicity reducing agents such as NaBH4 and ascorbic acid in the alkaline environment. Then, copper nanocluster is successfully synthesized with the excitation wavelength at 420 nm and the emission wavelength at 496 nm. It is deduced that Cu13 is the main product and uses its characteristics as a fluorescent probe. In the process, copper nanoclusters and hemoglobin were used as an IFE absorber/fluorophore pair and can be successfully used in real urine samples hemoglobin detection. This method is convenient, the detection limit of 70 nM. In addition, in different acid-base environments, the pepsin changes its structure due to the protonation and deprotonation of functional groups, resulting in a change in its fluorescence intensity. It can be used as a fluorescent probe to detect the change of pH value in the environment. The experimental results can be obtained in the linear range of pH 2.0 ~ pH 6.5, which can distinguish the difference of pH range of 0.5. After repeated regulation in the acid-base environment, they still keep high stability. After that, copper nanoclusters are used in the detection of urea, detection limit of 0.1mM, and can be successfully applied to the human urine sample. Finally, using laser scanning confocal microscopy to observe the copper nanoclusters, the fluorescence intensity of the copper nanoclusters showed an enhanced trend between pH 4.0 and pH 6.0, indicating that the copper nanoclusters have high cell penetration and high biocompatibility.
    Advisory Committee
  • Cheng-Ju Yu - chair
  • Tai-Chia Chiu - co-chair
  • Wei-Lung Tseng - advisor
  • Files
  • etd-0003118-150231.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2018-01-03

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