禁食為水產養殖業常見的操作程序。禁食被認為會影響魚類的生理調控機制與恆定。了解並評估禁食時間長短對魚類生理狀態的反應與變化，在水產養殖實務操作上十分重要。本研究將尖吻鱸(Lates calcarifer)進行禁食狀態下一天、四天與七天，進而以逆境反應指標探討尖吻鱸的生理狀態。本研究之逆境生理指標包含肝體比指數 (hepatosomatic index)、肝臟及肌肉中的肝糖含量；血糖、血清總蛋白量、血清滲透壓、血清鈉離子及氯離子濃度和熱休克蛋白 70 表現量。結果顯示，肝體比指數、肝臟之肝醣含量、血清總蛋白量與熱休克蛋白70 (HSP70)表現量, 在禁食後四與七天的狀態下，皆呈現下降趨勢，血清滲透壓在禁食七天後也有顯著性的下降；然而，肌肉之肝醣含量、血清中鈉離子及氯離子濃度在禁食後卻沒有顯著變化。本研究顯示，短期禁食緊迫會擾亂尖吻鱸的生理調節，此結果提供之相關指標，可用於評估禁食緊迫對養殖魚類的影響。

Fasting is a common process in aquaculture industry. Fasting was thought to influence physiological performance and homeostasis of fishes. To clarify and evaluate physiological changes through peroids of time in fish subjected to fasting is crucial for aquacultural practice. In this study, the Asian seabass (Lates calcarifer) were fasted for 1, 4 and 7 days to evaluate the physiological responses of fish. The secondary stress responses were examined, including hepatosomatic index (HSI), liver glycogen, serum glucose/total protein, serum osmolarity, concentrations of sodium and chloride ions and the expression of heat shock protein 70 (HSP70). The results showed that HSI, liver glycogen content and HSP70 expression, and serum total protein decreased significantly after fasting for 4 and 7 days (p<0.05). However, no change in serum glucose, [Na+] and [Cl-] was found. Our results revealed that fasting stress in 7 days disturbs multiple physiological responses of Asian seabass and provide related information for evaluating the effect of fasting stress on aquaculture fish species.

論文審定書........................................................................i
致謝…………………………………………………………………………………......ii
中文摘要………………………………………….……………………….…………..iv
英文摘要………………..…………………………………………………………….v
壹、前言…………………………………..……………….………………………….1
貳、材料與方法…………………………………………………………...………….8
一、物種飼養條件....................................................................................................9
二、實驗流程及採樣………………………………………………………………9
三、肝醣測定及肝體比計算……………………………………………………..10
四、血清參數分析………………………………………………………………..10
五、蛋白質萃取…………………………………………………………………..11
六、蛋白質定量…………………………………………………………………..11
七、蛋白質電泳…………………………………………………………………..12
八、免疫末點法…………………………………………………………………..12
九、統計方法……………………………………………………………………..13
參、結果……………………………………………………………………………..14
一、尖吻鱸短期禁食後肝體比的變化…………………………………………..14
二、尖吻鱸短期禁食後肝醣含量的變化………………………………………..14
三、尖吻鱸短期禁食後血液參數的變化………………………………………..15
四、尖吻鱸短期禁食後肝臟熱休克蛋白 70 的表現量………………………..15
肆、討論………………………………………………….………………………….17
一、禁食基準數據的評估………………………………………………………..17
二、能量代謝指標………………………………………………………………..17
三、血清逆境指標………………………………………………………………..18
四、細胞逆境指標………………………………………………………………..20
參考文獻……………………………………………..................................................22
圖次…………………………………………………………………….…………….32
Figure 1……………………………………………………………………………32
Figure 2……………………………………………………………………………33
Figure 3……………………………………………………………………………34
Figure 4……………………………………………………………………………35
表次…………………………………………………………………………………..36
Table 1……………………………………………………………………………..36
Table 2……………………………………………………………………………..37
Table 3……………………………………………………………………………..38
Table 4……………………………………………………………………………..39

行政院農業委員會漁業署，養殖漁業放養查詢平台 (民107年12月29日)。
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